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Objective To investigate the status quos of helicobacter pylori infection in people of the area bordering Yunnan,Guizhou and Guangxi so as to provide the basis for the prevention of diseases caused by helico-bacter pylori infection. Methods There were 29547 people including 6644 civil servants,1484 medical staff,5839 factory workers,5542 teachers,10038 students,who took physical examination from January,2012 to Decem-ber,2015 in the Affiliated Hospital of Youjiang Medical University for Nationalities. According to the principle of stratified sampling,3363 people were selected at random from the above groups. Among them there were 756 civil servants,215 medical staff,689 factory workers,654 teachers,1049 students. And there were 1515 Zhuang people, 897 Han people,234 Miao people,282 Yao people ,147 Gelao ,134 Buyi,154 people from other ethnics. Their ages ranged from 7 to 65. ELISA reagent method was adopted to detect helicobacter pylori infection. The relationship between ethnicity,gender,age,occupation,region and infection rate was analyzed. Results The total positive rate of helicobacter pylori was 47.2%in this region. As for different ethnic groups,the positive rate of Zhuang people was as high as 52.7%,and that of Kelao people was the lowest ,only 31.3%. Women′s infection rate was 41.5%,while that of the male was 52.4%. In terms of different age groups,people aged from 50 to 59 got an infection rate as high as 55.3%,while the age group under 18 had the lowest rate,only 19.6%. In the aspect of different professions,civil servants had the highest positive rate 57.9%. Students had the lowest rate 35.9%. Regional distribution analysis showed that Baise had the highest infection rate(51.4%),while Qian xi nan had the lowest rate of 33.9%. There was statistical difference in helicobacter pylori infection between different ethnic groups,genders,ages,occupations and regions(P<0.05). Conclusion The infection of helicobacter pylori in people of the area bordering Yunnan, Guizhou and Guangxi are related to ethnicity,gender,age,occupation and region.
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Objective To investigate the effect of artemisinin on the proliferation of human hepatoma cell line HepG‐2 .Methods The inhibition effect of cell proliferation in human hepatocelluar carcinoma cell line HepG2 of artemisinin was detected by MTT test ,and the cell cycle and apoptosis were detected by Flow cytometry .Results Artemisinin at 80 umol/L could effectively inhibi‐ted the proliferation of HepG‐2 cell in a dose‐and time‐dependent manner;the drugs could block cells at G0/S phase ,and induct the HepG‐2 cell apoptosis .Conclusion Artemisinin could effectively inhibit the proliferation of HepG‐2 cell .
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<p><b>OBJECTIVE</b>To investigate the effect and molecular mechanism of cisplatin (DDP) combined with Matrine (Ma;plant alkaloid) against hepatocellular carcinoma using a nude mouse model with xenografted human tumors.</p><p><b>METHODS</b>Twenty-four 6-week old male BALB/c nude mice were subcutaneously injected with HepG2 cells into the axilla, and randomly divided into four groups:control (NS) group,Ma treatment group,DDP treatment group and DDP+Ma combination treatment group. All treatments were delivered via intraperitoneal injection.Changes in whole body weights and tumor volume were assessed by before and after treatment measurements and plotting of growth curves. After 14 days of drug intervention, the mice were sacrificed for collection of tumor tissue and assessment of the tumor inhibition rates for each treatment. Affects on expression of survivin and caspase-3 were assessed by immunohistochemistry. ANOVA test and t-test were performed for the statistical analyses.</p><p><b>RESULTS</b>The tumor inhibition rates for the various treatments were:37.5%,Ma alone;75.0% DDP alone;83.3%,DDP+Ma group DDP combined. The DDP+Ma-induced inhibition was significantly greater than that achieved wit Ma or DDP alone (both P less than 0.05). The average weight of the DDP+Ma group (21.5 g) was lower than that of the NS group (28.5 g) and the Ma group (26.67 g),but higher than that of the DDP group (17.33 g).In addition, the DDP+Ma group also showed more robust general health,as indicated by activity,participation in life routines and appetite,than the DDP group. The rate of positive staining for survivin expression in tumor tissues was significantly lower in the DDP+Ma group (19.58%+/-4.52%) than in the NS group (83.26%+/-15.56%), the Ma group (62.50%+/-8.09%), and the DDP group (38.67%+/-8.26%) (all P less than 0.05).In contrast, the rate of positive staining for Bax expression was significantly higher in the DDP+Ma group (78.26%+/-6.09%) than in the NS group (21.15%+/-3.68%), the Ma group (35.13%+/-10.57%), and the DDP group (65.88%+/-4.81%) (all P less than 0.05).</p><p><b>CONCLUSION</b>Treatment with Ma alone or DDP alone is sufficient to inhibit the growth ofxenografted human hepatocellular carcinoma cells in nude mice. The DDP+Ma combination treatment,however,shows greater inhibitory effect,suggesting that Ma may enhance DDP's anticancer properties. The improved health status of mice treated with DDP+Ma suggests that Ma may reduce DDP toxicity. The mechanism underlying these beneficial treatment effects may involve modulation of survivin/caspase-3 expression and subsequent apoptosis.</p>
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Animals , Humans , Male , Mice , Alkaloids , Pharmacology , Apoptosis , Carcinoma, Hepatocellular , Drug Therapy , Metabolism , Caspase 3 , Metabolism , Cisplatin , Pharmacology , Hep G2 Cells , Inhibitor of Apoptosis Proteins , Metabolism , Liver Neoplasms , Drug Therapy , Metabolism , Mice, Inbred BALB C , Mice, Nude , Neoplasm Transplantation , Quinolizines , Pharmacology , Tumor BurdenABSTRACT
Objective To study the genotyping of clarithromycin-resistant Helicobacter pylori(Hp) isolates.Methods From March 2007 to October 2007,247 gastric mucosa specimens were collected by the endoscopy from the patients with peptic ulcer or gastritis at the affiliated hospital of Youjiang Medical College for Nationalities.A total of 126 Hp strains were isolated.Resistance to clarithromycin in Hp was determined by E-test.All of the resistant isolates were genotyped with REP-PCR and further clustered with NTsys_2 software.The clinical data was collected for these patients with clarithromycin-resistant Hp.Results The 26 clarithromycin-resistant isolates from the west of Guangxi were divided into six genotypea including Group Ⅰ,Group Ⅱ,Group Ⅲ,Group Ⅲ,Group Ⅳ,Group Ⅴ and Group Ⅵ according to the homology of 78%.Every group have 2,11,1,8,3,1 strains Hp,respectively.Strains of group Ⅱ were isolated from patients with peptic ulcer and most of them from Chuang patients.All strains of group Ⅳ were isolated from patients with gastritis.Conclusions The clarithromycin-resistant isolates were divided into six groups by REP-PCR Disease type.nationality of patients and family history of stomach diseases were associated with the genotypes.
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objective To analyze the prevalence of Helicobacter pylori(Hp)strains resistant to clarithromycin and the relation of 23S rRNA genetic mutation with clarithromycin resistance.Methotis Hp strains were isolated from gastric mucosa biopsies of patients with peptic ulcer or gastritis.Resistanee of the isolates to elarithromycin wag determined using diffusion test.Mutations in elarithromyein resistant strains were identified by PCR-RFLP and gene sequencing.Results The prevalence of Hp strains resistant clarithromyein was 22.2%.10 of clarithromycin resistant strains had A2143G.A2144G point mutation in 23S rRNA gene,and none of the 10 susceptible strains had the A2143G or A2144G mutation,and the result was affirmed by measure sequencing.Conclusion The prevalence of Hp is higher than Beijing and Shanghai,mutations in 23S rRNA are associated with elarithromyein resistance in Hp.
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Objective To investigate the value of pleural effusion and C-reactive protein in severity evalua-tion and prognosis of acute pancreatitis. Methods Clinical data of 108 patients with acute pancreatitis were ana-lyzed retrospectively. AP classification was conducted on the basis of intensified CT examination and the clinical in-dexes including blood calculus, blood lipid, blood glucose and blood gas were measured. The criteria for diagnosis and severity evaluation of acute pancreatitis were based on results of chest X-ray, CT examination and CRP. The correla-tion between pleural effusion, CRP and the severity, etiology, prognosis of acute pancreaitis were analyzed. Results Of the 108 patients, mild acute pancreatitis (MAP) was found in 57 patients, and severe acute pancreatitis (SAP) in 51. Among SAP, 32 patients (62.75% ) developed pleural effusion, 38 patients (74.51% ) whose CRP > 20.00 mg/L,and 27 patients (52. 94%) had both pleural effusion and CRP > 20. 00 mg/L. There were 13 (22.81% ), 14 (24.56%) and 8 (14.04%) respectively among MAP. The difference between the two groups was sig-nificant(P<0.01). Among acute biliarv pancreatitis,9 patients (33.33%) developed pleural effusion and 15 (55.65%) whose CRP>20 mg/L and 9 (33.33%) had beth pleural effusion and CRP>20.00 mg/L; Among acholic AP,25 (65.79%) developed pleural effusion and 20 (52.63% ) whose CRP > 20.00 mg/L and 23 (60.53%) had beth pleural effusion and CRP>20.00 mg/L. Case fatality was also significantly different between group with pleural effusion,CRP >20.00 mg/L,both pleural effusion and CRP>20.00 mg/L,and group with non-pleural effusion,CRP≤20.00mg/L,non-pleural effusion or CRP≤20.00mg/L respectively(P<0.01). Conclusion Either pleural effusion or CRP can be used as independent prognostic parameters for severe acute pancreatitis,and the combined use of these two parameters is the most reliable.
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BACKGROUND: Based on the multi-directional differentiation potential,the combined application of proper biological materials and growth factor can repair tissue defect.OBJECTIVE: To observe the growth characteristics of rabbit bone marrow-derived mesenchymal stem cells and their osteogenic potential under the induced condition.DESIGN: A single sample experiment.SETTING: The Department of Histology and Embryology of Youjiang Medical College for Nationalities; the Affiliated Hospital of Youjiang Medical College for Nationalities.MATERIALS: This experiment was conducted at the Department of Histology and Embryology of Youjiang Medical College for Nationalities in June 2004. Male new Zealand rabbits, with a body mass of 2.0 to 2.5 kg,were provided.METHODS: Rabbit bone marrow tissue was extracted. After gradient centrifugation, adherent cells were kept for cell passage. Culture medium was used for inducing culture of osteoblast when the passage was stable (50 mL/L of Dulbecco modified culture medium of bovine serum containing 10 mmol/L of β-sodium glycerophosphate, 10 nmol/L of dexamethasone, 50 mg/L of vitamine C). The culture medium was changed every other day. Osteogenic characteristics of the bone marrow-derived mensenchymal stem cells were observed under an inverted microscope after alkaline phosphatase immunohistological staining, osteonectin and osteopontin immunocytochemical staining.MAIN OUTCOME MEASURES: ① Morphological observation of bone marrow-derived mesenchymal stem cells; ② Osteogenic characteristics of bone marrow-derived mesenchymal stem cells.RESULTS: ① Morphological observation of bone marrow-derived mensenchymal stem cells: Primary rabbit bone marrow-derived mesenchymal stem cells could cover the Petri dish fully in 7 or8 days and could passage stably. Generative cells began to passage in 5 or 6 days. ② Osteogenic characteristics of bone marrow-derived mensenchymal stem cells:Alkaline phosphatase immunohistochemical staining showed a lot of brown particles in the kytoplasm, but no colored cells were found in the control group. Osteonectin and osteopontin immunohistochemical detection found that the nucleus was light blue and a lot of brown particles appeared in the kytoplasm, presenting obvious strong positive, but no brown particles appeared in the control group.CONCLUSION: After combined induced culture with dexamethasone, vitamine C and β-sodiumglycerophosphate, rabbit bone marrow mensenchymal stem cells showed the morphological and biological characteristics of osteoblasts with osteoblastic activity, and were able to supply autologous seed cells for constructing in vitro tissue engineered bone in a short period of time.
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BACKGROUND: Polymorphism of exon 5 +3953C/T of interleukin-1β (IL-1β) gene is found in some studies. This gene polymorphism is related to stomach cancer, rheumatism, arthritis and other diseases. However, what is the distribution property of genotype of IL-1β gene in Guangxi Zhuang population? OBJECTIVE: To observe the distribution of the polymorphism of exon 5 +3953C/T of 1L-1β gene in Chinese Guangxi Zhuang population and Han population, and compare its distribution with Caucasians and African white people.DESIGN: Contrast observation.SETTING: Department of Histology and Embryology,Youjiang Medical College for Nationalities.PARTICIPANTS: Persons who received the health examination in the Affiliated Hospital of Youjiang Medical College for Nationalities were recruited in the experiment. Among them, 155 were Zhuang nationality, including 80 male and 75 female, aged (37) years, and 195 were Han na tionality, including 100 male and 95 female, aged (38) years. Blood relationship was not found among them,and informed consents were obtained from all the participants.METHODS: This experiment was carried out in the Experimental Center for Medical Sciences,Guangxi Medical University from June 2003 to May 2005. Polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) technique was used to detect the polymorphism of exon 5 +3953C/T of IL-1β gene in Guangxi Zhuang population (n=155) and Han population (n=195).Genotype and allele frequency of IL-1β gene of healthy population were directly calculated with counting method, and they were compared between Caucasians and African white people combining with literatures.MAIN OUTCOME MEASURES: Comparison of genotype frequency and allele taking frequency of IL-1β gene (+3953) among different ethnic populations.RESULTS: Subjects who received healthy examination including 155 Guangxi Zhuang nationality and 195 Chinese Han nationality participated in result analysis. ①The frequencies of genotypes and allele of IL-1β gene (+3953C/T) were close between Guangxi Zhuang population and Han nationality (Frequency of genotype CC,CT TT and allele C and T of Zhuang population was 94.8％,5.2％,0,97.4％,2.6％,respectively,and that of Han population was 93.3％,6.7％,0,96.7％,3.3％,respectively,P＞0.05).②There were significant differences in genotype distribution and allele taking frequency of IL-1β gene between Caucasians,African white people and Guangxi Zhuang population,Han population (Frequency of genotype CC,CT TT and allele C and T of Caucasians was 52.2％,43.9％,3.9％,74.1％,25.9％,respectively,and that of African white people was 60.4％,33.2％,6.4％,76.4％,23.6％, respectively, P＜0.01).CONCLUSION: The distribution of polymorphism of exon 5 +3953C/T of IL-1β gene is close between Guangxi Zhuang population and Han population,but significant difference exists in comparison with Caucasians and African white people.
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Objective To compare the effects of endoscopic probe and self-made balloon device in dilation of the cardia for patients with cardia achalasia.Methods From January 1998 to December 2007,45 patients with cardia achalasia received gastroscopy-assisted dilation at our hospital;22 of them were treated by self-made balloon device and 23 were by endoscopic probe.The efficacies of the two methods were compared.Results In the probe group,each patients received 1 to 9 times of dilation(mean,3.6),while in the balloon group it was 1 to 6 times(mean,2.3).None of the two groups had intra-and post-operative complications.The cost for the first treatment was(1542.57?281.30) yuan in probe group,and(861.91?176.48) yuan in the balloon group(t=9.671,P=0.000).The efficacy of the two groups was similar in 3 months after the treatment [the rates of the cured,improved,and failed were 61%(14),35%(8),and 4%(1) in the probe group,and 68%(15),23%(5),and 9%(2) in the balloon group,Z=-0.351,P=0.726],but significantly different in 6 months [22%(5),13%(3),and 65%(15) vs 45%(10),32%(7),and 23%(5),Z=2.564,P=0.010].Conclusions Both endoscopic probe and self-made balloon device are effective in dilatation of the cardia for cardia achalasia.Self-made balloon device dilatation is exceptionally suitable for local hospitals because of its low cost and simple techniques.